Ribosome-bound Polyriboadenylate Polymerase
نویسندگان
چکیده
منابع مشابه
Structure of Escherichia coli Hfq bound to polyriboadenylate RNA.
Hfq is a small, highly abundant hexameric protein that is found in many bacteria and plays a critical role in mRNA expression and RNA stability. As an "RNA chaperone," Hfq binds AU-rich sequences and facilitates the trans annealing of small RNAs (sRNAs) to their target mRNAs, typically resulting in the down-regulation of gene expression. Hfq also plays a key role in bacterial RNA decay by bindi...
متن کاملPurification and properties of a ribonucleic acid primer-independent polyriboadenylate polymerase from Escherichia coli.
An enzyme isolated from Escherichia coli catalyzes the synthesis of polyriboadenylate from ATP in the absence of a RNA primer. The reaction is dependent on the presence of either Mg 2+ or Mn*+, 0.8 or 0.2 mu being optimal, respectively. Addition of Mg2f to reaction mixtures containing optimal concentration of Mn2+ or vice versa results in marked inhibition of the polymerization reaction. Inclus...
متن کاملRibonucleic Acid-dependent Ribonucleotide Incorporation. Ii. Inhibition of Polyriboadenylate Polymerase Activity following Bacteriophage Infection.
Synthesis of polyriboadenylate is accompanied by the concomitant release of inorganic pyrophosphate. Under appropriate conditions, poly Al is formed in excess of RNA added to the reaction mixture. The dependence of the reaction on RNA is shown (a) by complete loss of poly A synthesis in the presence of pancreatic RNase, although the reaction product is highly resistant to this RNase, and (b) by...
متن کاملDirect evidence that ribosome bound RNA-dependent RNA polymerase does not play a role in globin messenger RNA replication.
The radioactively labelled product of RNA-dependent RNA polymerase+ from ribosomes of immature chicken erythrocytes was tested for the presence of newly replicated globin mRNA using unlabelled globin complementary DNA. No radioactively labelled globin mRNA sequences were found in the product, providing direct confirmation that this RNA-dependent RNA polymerase is not involved in globin mRNA amp...
متن کاملDksA guards elongating RNA polymerase against ribosome-stalling-induced arrest.
In bacteria, translation-transcription coupling inhibits RNA polymerase (RNAP) stalling. We present evidence suggesting that, upon amino acid starvation, inactive ribosomes promote rather than inhibit RNAP stalling. We developed an algorithm to evaluate genome-wide polymerase progression independently of local noise and used it to reveal that the transcription factor DksA inhibits promoter-prox...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1966
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)99863-7